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. 2012 Mar 20;7(3):e33152. doi: 10.1371/journal.pone.0033152

Figure 2. Examination of CD69-negative SP thymocytes in WT and IL-15Rα KO mice.

Figure 2

Thymocytes of WT and IL-15Rα KO mice (n = 6) were immunostained for CD4, CD8, TCR, and CD69. The percentages of CD69-negative (A) and CD69-positive (B) CD8SP TCRhi (left panels) and CD4SP TCRhi thymocytes (right panels) among total TCRhi cells were compared. By calculation, the cell numbers of CD69− CD8SP TCRhi thymocytes in WT and IL-15Rα KO mice were about 1.6×106±3.8×105 and 9.0×105±2.8×105, respectively. The cell numbers of CD69− CD4SP TCRhi thymocytes in WT and IL-15Rα KO mice were about 3.6×106±1.3×105 and 3.2×106±1.4×105, respectively. Data are presented as means ± SD and were analyzed by single-classification ANOVA. *p<0.05; n.s., not significant.