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. 2012 Mar 20;7(3):e33152. doi: 10.1371/journal.pone.0033152

Figure 4. In vivo proliferation of CD8SP TCRhi and CD4SP TCRhi thymocytes analyzed by BrdU incorporation.

Figure 4

WT and IL-15Rα KO mice (n = 7) were injected i.p. with BrdU. Total thymocytes were isolated 1 hr later, and the degrees of BrdU incorporation by CD4SP TCRhi and CD8SP TCRhi cells was determined. (A) Upper panel; The BrdU incorporation of IL-15Rα KO CD8SP TCRhi cells (3.2×104±1.1×103 cells) was compared with that of WT CD8SP TCRhi cells (9.4×104±3.1×103 cells). Lower panel; The percentages of WT (1.6×105±1.0×105 cells) and IL-15Rα KO CD4SP TCRhi (1.5×105±0.7×105 cells) thymocytes that incorporate BrdU. (B) Values of each experimental group are averaged and the means are indicated by horizontal lines. Data are presented as means ± SD. **p<0.005; n.s., not significant. Single-classification ANOVA.