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. 2012 Mar 20;7(3):e33152. doi: 10.1371/journal.pone.0033152

Figure 5. In vitro stimulation of CD8SP thymocyte proliferation with plate-bound IL-15-IL-15Rα-Fc proteins.

Figure 5

Total thymocytes were labeled with CFSE and then cultured in medium supplemented with IL-15, IL-21, both IL-15 and IL-21, or both IL-15 and IL-21 with pre-coated rmIL-15Rα-Fc fusion protein. The cultures were supplemented with the same amounts of cytokine 3 days later. Cells were harvested 5 days after the initiation of the culture and stained for CD4 and CD8. The extent of cell proliferation in CD8SP thymocytes was analyzed by measuring CFSE dilution. The data are representative of three independent experiments.