Figure 6. The dependence of CD8⁺ RTEs on IL-15 transpresentation.

Mice were injected twice i.p.with BrdU at a 2-hr interval. Four hours after the second BrdU injection, mice were injected intrathymically with FITC or subjected to a sham operation (n = 6). (A) Cells were collected from the LNs 16 hours later and immunostained for the expression of CD8 (left panels) and CD4 (right panels). (B) The frequencies of BrdU⁺ FITC⁺ CD8⁺ or BrdU⁺ FITC⁺ CD4⁺ LN cells among FITC⁺ RTEs were compared. **p<0.005; n.s., not significant.