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. 2012 Mar 20;7(3):e33750. doi: 10.1371/journal.pone.0033750

Figure 8. ALG-1/2 function is required to maintain muscles and the epidermis.

Figure 8

(A–B) Embryos collected between 6–8 hours post egg-laying at 25°C and stained with the antibodies MH27 (adherens junction). The alg-1; alg-2 mutant embryo did not progress beyond the two-fold stage, yet has grossly normal junctions. (C–E) Embryos collected between 6–8 hours post egg-laying at 25°C and stained with the antibodies 4F2 (VAB-10A; C–E) and NE8/4C6 (muscle; C′–E′); merge picture (C″–E″). Arrow, are where the fibrous organelle (D–E) and muscle (D′–E′) pattern is partially interrupted; in addition, muscles do not closely follow the body wall in this area (they should be closer to the blue dotted line; see blue arrowheads). Embryos did not elongate beyond the two-fold stage.