Table 1. Methods used for detection and molecular characterization of the samples from cases of of acutegastroenteritis in a day-care center, Rio de Janeiro, Brazil, 1994–2008.

Viruses	Virusesdetection		Virusescharacterization
	Methods	References	Methods/region	References
RVA	PAGE, EIA	[25], IDEA® Rotavirus *or RIDASCREEN® Rotavirus **	Semi-nested multiplex PCR, VP4 (G)/VP7(P)	[26] [27] [24]
NoV	Real time PCR	[28]	Region B(RpRd), Region C (Capsid),Region D (Capsid)	[29] [30] [31]
AstV	Real time PCR	[32]	ORF2	[33]

^*OXOID Ltd, England.

^**R Biopharm Group, Germany.