Figure 4. CB2 agonism inhibits SDF-1á mediated G-protein coupling to CXCR4.
(A–B) GTPãS binding following addition of the CXCR4 agonist SDF-1á to permeabilized primary CD4+ T cells pretreated with either DMSO or 100 nM of the CB2 agonist JWH-133 (A) in a representative donor and (B) in multiple donors (mean±SEM; n = 4 donors; **p = 0.0034, *p<0.02). (C–D) GTPãS binding following addition of the CB2 agonist JWH-133 to permeabilized CD4+ T cells pretreated with either DMSO or 100 nM of the CXCR4 agonist SDF-1á (C) in a representative donor and (D) in multiple donors (mean±SEM; n = 4 donors).