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. 2012 Mar 20;7(3):e34123. doi: 10.1371/journal.pone.0034123

Figure 6. Expression of the sensory epithelium marker Parvalbumin and Sox2 in new HCs, and Sox10 in new SCs.

Figure 6

(A–A′) Images of samples double stained with Parvalbumin and EGFP at HC layer (A) and SC layer (A′) of the ectopic sensory patches in the utricle non-sensory region of a CAGCreER+; Rosa26-NICDloxp/+ embryo treated with tamoxifen at ∼E13 and analyzed at ∼E19. The arrow points to a new Parvalbumin+ HC. (B–B′) Triple staining of Myosin-VI, Sox2, and EGFP. Both ectopic HCs (B) and SCs (B′) were Sox2+. The arrow points to a new Sox2+/Myosin-VI+ HC. Of note, Myosin-VI was visualized in a pseudo-green color. (C–C′) Triple staining of Myosin-VI, Sox10, and EGFP. The arrow points to a new Myosin-VI+/Sox10−negative HC. The SCs (either EGFP+ or EGFP−negative) were Sox10+. Note that Myosin-VI was also visualized in a pseudo-green color. Scale bars: 20 µm.