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. Author manuscript; available in PMC: 2013 Mar 10.
Published in final edited form as: Steroids. 2011 Dec 8;77(4):300–305. doi: 10.1016/j.steroids.2011.12.003

Figure 2.

Figure 2

(A) Representative EMSA bands from the two study groups showing the quantity of NFκB in nuclear extracts from mononuclear cells (MNC) in samples collected in the fasting state (0) and 2 hours post-glucose ingestion (2), before and after treatment with DHEA or placebo. Densitometric quantitative analysis comparing the change from baseline (%) in MNC-derived activated NFκB between the two study groups for fasting samples before and after (before versus after, 0) DHEA or placebo administration (left panel); and for fasting and 2 hour post-glucose ingestion samples for each OGTT (before, 0 versus 2; after, 0 versus 2) as a measure of the response to glucose challenge before and after DHEA or placebo administration (right panel). After DHEA administration, the % change in activated NFκB was significantly greater compared to placebo in the fasting state (*, P<0.04), and in response to glucose ingestion (†, P<0.005). (B) Comparison between groups of the change from baseline (%) in TNFα mRNA content in MNC for fasting samples before and after (before versus after, 0) DHEA or placebo administration (left panel); and for fasting and 2 hour post-glucose ingestion samples for each OGTT (before, 0 versus 2; after, 0 versus 2) as a measure of the response to glucose challenge before and after DHEA or placebo administration (right panel). Values are normalized to 28S rRNA expression. After DHEA administration, the percent (%) change in TNFα mRNA transcripts significantly increased compared to placebo in the fasting state (*, P<0.05), and in response to glucose ingestion (†, P<0.05). Adapted from González et al. [43], with permission. Copyright The American Physiological Society, 2011.