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PCR for Neospora caninum using TIM11-LAV1 primers (TIM11 by Payne & Ellis, 1996. LAV1 produced by the authors). Lanes (1) Positive control, NC-1 isolate in Vero cells (588 bp); (2) and (3) Fetal bovine brain from a case of neosporosis unrelated to this report; (4) 50-bp DNA ladder (Invitrogen); (5) and (6) Caprine placenta from the case reported in these paper; (7) Negative control, MDBK cells DNA.
