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Semi-quantitative RT-PCR for CREB-1 isoforms and ICER was performed in triplicate. Activated T cells show higher levels of both CREB-1 isoforms, when compared to naïve T cells, whereas naïve T cells show higher levels of ICER then activated T cells. Jurkat T leukaemia cells show virtually undetectable levels of ICER. Treatment of Jurkat cells with Zebularine, DZNep and MS275 does not influence CREB-1 or ICER transcript levels.
