Table 1.

Sequences of primers and probes used to detect HPV DNA by quantitative polymerase chain reaction

HPV-16 E6

Forward primer, 5′-TCAGGACCCACAGGAGCG-3′

Reverse primer, 5′-CCTCACGTCGCAGTAACTGTTG-3′

Probe, 5′-(FAM)- CCCAGAAAGTTACCACAGTTATGCACAGAGCT-(TAMRA)-3′

HPV-16 E7

Forward primer, 5′-CCGGACAGAGCCCATTACAA-3′

Reverse primer, 5′-CGAATGTCTACGTGTGTGCTTTG-3′

Probe, 5′-(FAM)-CGCACAACCGAAGCGTAGAGTCACACT-(TAMRA)-3′

β-actin

Forward primer, 5′-TCACCCACACTGTGCCCATCTACGA-3′

Reverse primer, 5′-CAGCGGAACCGCTCATTGCCAATGG-3′

Probe, 5′-(FAM)-ATGCCCTCCCCCATGCCATCCTGCGT-(TAMRA)-3′