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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Arch Toxicol. 2011 Nov 22;86(4):633–642. doi: 10.1007/s00204-011-0778-y

Fig. 6. Tfam and mtDNA-encoded target gene mRNA expression is increased in response to treatment with E2, 4-OHT, and RAL.

Fig. 6

HUVECs were treated with 10 nM E2, 100 nM 4-OHT, or 100 nM RAL. Where indicated, cells were also pretreated for 6 h with 100 nM ICI 182,780 followed by addition of 10 nM E2, 100 nM 4-OHT, or 100 nM RAL for 48 h. Total mRNA was isolated following treatment and QRT-PCR was performed as described in Material and Methods. Values are the average of 3 separate experiments ± SEM. TFAM is nuclear-encoded and regulated by NRF-1. Tfam regulates mtDNA-encoded target genes cytochrome c oxidase subunit I (COI, MTCO1); and NADH dehydrogenase subunit I (NDI). *, p < 0.05 versus EtOH control; ** p < 0.05 versus the same treatment without ICI.