Fig. 1.

Quantitative extracted lipid yields (as % DW) of Nannochloropsis sp. biomass by gravimetric determination after solvent extraction using six different procedures, four of which used an accelerated solvent extractor (ASE) with different temperature and pressure conditions. All data shown are the mean ± SD of triplicate extractions, from L to R: ASE.1 40 °C 500 psi with chloroform/methanol (C:M); ASE.2 100 °C 2,000 psi C:M; ASE.3 40 °C 500 psi with hexane/IPA (HI); ASE.4 100 °C 2,000 psi with HI; Soxhlet.1 16 h Soxhlet extraction with CM; Soxhlet.2 3 h Soxhlet extraction with CM followed by extract washing