Table 3.

Peptides from the digest of both BoNT/B1 and /B2 with amino acid differences that were discovered through the use of the amino acid substitution database

Amino acid position	Difference (B2 Prevot 25)	Normal (B1 Okra)
472-484	IEYNTQSNYIENR	IEYNTQSNYIEND
599-610	DFVIEANKSSTM	DFVIEANKSNTM
850-868	EIFNKYNSEILNNIILNLR	EMFNKYNSEILNNIILNLR
876-887	DLSGYGANVEVY	DLSGYGAKVEVY
910-923	VTQNQNIIFNSMFL	VTQNQNIIFNSVFL
970-980	IIWTLTDINGK	IIWTLIDINGK
976-992	DINGKTKSVFFEYSIRE	DINGKTKSVFFEYNIRE
1057-1071	YFSIFNTELSQSNIK	YFSIFNTELSQSNIE
1072-1084	EIYKIQSYSEYLK	ERYKIQSYSEYLK
1114-1123	DSSVGEILTR	DSPVGEILTR