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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Mol Genet Metab. 2012 Jan 5;105(4):652–657. doi: 10.1016/j.ymgme.2011.12.021

Figure 2. Effect of BVVLS missense mutations on RF uptake.

Figure 2

RF uptake by human intestinal epithelial Caco-2 cells transiently transfected with wild-type (WT) and mutated hRFT-2. Uptake of 3H-RF (14 nM) was measured 48 hours post transfection in Krebs-Ringer buffer (pH 7.4) as described in MATERIALS AND METHODS and expressed in fmol·mg protein−1·2 min−1. Data are means ± SE of 3–5 independent experiments. GFP - cells transiently transfected with the empty GFP-C3 vector. *P < 0.01.