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. Author manuscript; available in PMC: 2012 May 1.

Published in final edited form as: Prostate. 2011 Jul 27;72(6):612–625. doi: 10.1002/pros.21464

Fig. 1 — Structure of ca27 and down-regulation of AR protein expression in prostate cancer cell lines. ca27 (1,5-Bis(2-hydroxyphenyl)-1,4-pentadien-3-one) (A) consists of two phenolic rings with symmetrical hydroxyl groups on the ortho position of the aryl rings, which are linked by an unsaturated 5-carbon spacer with a single carbonyl. The synthesis of ca27 was previously described in Weber et al. 2006 [14]. Down-regulation of AR protein expression ca27 in LNCaP, C4-2 and LAPC-4 cells. LNCaP (B), C4-2 (C) and LAPC-4 (D) cells were treated with 1 and 5 μM ca27. AR protein was measured by western blotting and densitometric analysis (ratio AR: β-actin) after 12 hours. LNCaP (E) cells were treated with 20 μM ca27 or curcumin for 72 hours AR protein expression was measured and quantitated as described above. LNCaP (F) cells were pretreated with 10 μ MMG132f or 1 hour before the addition of 5 μM ca27 for 6 hours. One representative western blot is shown; bars in the graph represent the average of triplicate values + standard deviation. * denote P < 0.05comparedto control.

Fig. 1 — Structure of ca27 and down-regulation of AR protein expression in prostate cancer cell lines. ca27 (1,5-Bis(2-hydroxyphenyl)-1,4-pentadien-3-one) (A) consists of two phenolic rings with symmetrical hydroxyl groups on the ortho position of the aryl rings, which are linked by an unsaturated 5-carbon spacer with a single carbonyl. The synthesis of ca27 was previously described in Weber et al. 2006 [14]. Down-regulation of AR protein expression ca27 in LNCaP, C4-2 and LAPC-4 cells. LNCaP (B), C4-2 (C) and LAPC-4 (D) cells were treated with 1 and 5 μM ca27. AR protein was measured by western blotting and densitometric analysis (ratio AR: β-actin) after 12 hours. LNCaP (E) cells were treated with 20 μM ca27 or curcumin for 72 hours AR protein expression was measured and quantitated as described above. LNCaP (F) cells were pretreated with 10 μ MMG132f or 1 hour before the addition of 5 μM ca27 for 6 hours. One representative western blot is shown; bars in the graph represent the average of triplicate values + standard deviation. * denote P < 0.05comparedto control.