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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Prostate. 2011 Jul 27;72(6):612–625. doi: 10.1002/pros.21464

Fig. 6.

Fig. 6

Nrf2activation and up-regulation ofNrf2 regulated genes in LNCaP and LAPC-4cellsby the synthetic curcumin analog ca27. LNCaP (A) and LAPC-4 (B) cells were co-transfected with Nrf2 reporter plasmid driving luciferase and TK reporter plasmid driving Renilla luciferase. Normalized luciferase activity was determined 16 hr post-treatment with 1 and 5 μM ca27. Bars represent the average of quadruplicate values + SD. Nrf2 activation is expressed as % of control. LNCaP cells (C) were treated with vehicle control or 5 μM ca27 for 3 hr. NQO1, AKR1C1, and MafG mRNA expression was measured by qRT-PCR. Bars represent the average of triplicate values + SD. NQO1, AKR1C1and MafG expression normalized to GAPDH is shown relative to control. * denotes P < 0.05, respectively compared to control.