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. Author manuscript; available in PMC: 2013 Mar 23.
Published in final edited form as: FEBS Lett. 2012 Jan 27;586(6):717–721. doi: 10.1016/j.febslet.2012.01.024

Figure 3. In vitro tRNA selenation by tRNA 2-selenouridine synthase.

Figure 3

E. coli YbbB (lane 1), MMP0899 (lane 2), MMP0900 (lane 3) or MMP0899 and MMP0900 (lane 4) were incubated with total tRNAs from the M. maripaludis Mm900 ΔselU strain and [75Se]selenophosphate to generate selenated tRNAs. tRNAs were extracted from the reaction and resolved by 12% Urea-PAGE, followed by autoradiography. Migration position of 75Se-labeled tRNAs is indicated on the left.