Figure 3.

Maximum likelihood phylogenetic tree based on aligned partial amino-acid sequences of the mcrA. Amino-acid composition was deduced from DNA sequences, and the tree was calculated with RAxML 7.04 using rapid hill climbing algorithm and PROTMIX-JTT evolutionary model. Bootstrap values above 50% (out of 100 trials) are displayed next to the nodes. Shaded clusters contain sequences that were only detected in the preincubated soil samples, whereas sequences from the post-incubated slurries cluster both into the shaded clusters and into the shaded clusters with diagonal lines clusters.