Figure 4.

Localisation of bacteria in moss gametophytes. Fluorescent in situ hybridisation (FISH) of S. fallax leaves showed colonisation of the outer surface (a and b) and hyaline cells (c and d). Violet: cell walls of Sphagnum cells; green: chlorophyll-containing Sphagnum chlorocytes; yellow: Alphaproteobacteria; red: other bacteria. Images acquired by confocal laser scanning microscopy (CLSM; panels a and c) and processed by 3D computer reconstruction using Imaris7.0 (b and d). Scale bar=10 μm.