Table 3. Number of T-RFLP fragments and % of fluorescence contributed by major bacterial phyla (for which TRFs could be assigned).
| Number of Fragments | % Cyanobacteria (pk 430) | % Actinobacteria (pk 76-79, 162-164) | % Chloroflexi (pk 100-101, 156) | % Bacteriodetes (pk 69, 110, 293) | % alpha-Proteobacteria (pk 415-416, 417-418) | |
|---|---|---|---|---|---|---|
| (A) Arches | ||||||
| Untrampled control | 4 (1) a | 60 (5.1) a | 0 (0) a | 0 (0) a | 0 (0) a | 19 (4.7) a |
| Trampled (1 month ago) | 16 (2.1) b | 46 (7.3) a | 19 (6.0) b | 5 (0.5) b | 2 (0.3) b | 13 (1.2) a |
| Trampled (1 year ago) | 13 (2.3) b | 40 (5.9) a | 11 (5.4) b | 4 (2.4) b | 2 (0.5) b | 13 (4.6) a |
| F-test result, P-value | 0.0002 | 0.0982 | 0.0038 | 0.0099 | 0.0002 | 0.1500 |
| (B) ISKY-1 | ||||||
| Untrampled control | 3 (1.6) a | 85 (2.8) a | 0 (0) a | 3 (1.3) a | 0 (0) a | 0 (0) a |
| Trampled (1 month ago) | 14 (1.6) b | 28 (24.5) b | 19 (5.5) b | 7 (1.3) a | 3 (0.7) b | 7 (2.0) b |
| Trampled (1 year ago) | ND | ND | ND | ND | ND | ND |
| T-test result, P-values | 0.0033 | 0.0037 | 0.052 | 0.075 | 0.030 | 0.041 |
| (C) ISKY-2 | ||||||
| Untrampled control | 7 (1.5) a | 88 (5.1) a | 2 (1.5) b | 1 (0.7) a | 1 (0.5) a | 3 (1.7) a |
| Trampled (1 month ago) | 17 (7) b | 31 (11.0) b | 17 (8.7) a | 8 (2.8) b | 7 (2.9) b | 10 (11.6) a |
| Trampled (1 year ago) | 17 (1) b | 52 (5.6) c | 10 (2.8) ab | 7 (3.3) b | 4 (1.1) b | 11 (6.5) a |
| F-test result, P-value | 0.0068 | 0.0001 | 0.0093 | 0.0116 | 0.0023 | 0.3508 |
Abbreviations: ND, not determined; pk, T-RFLP peak or fragment length.
P-values are from one-way analysis of variance with three treatments (for Arches, ISKY-2 sites), or with two treatments (for ISKY-1 site). Values in bold text followed by different letters were significantly different (alpha =0.05 or less) by Tukey's HSD mean separation procedure (Arches, ISKY-2 sites, or by pair-wise t-test (ISKY-1 site).