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. Author manuscript; available in PMC: 2012 Dec 15.
Published in final edited form as: J Immunol. 2011 Nov 11;187(12):6374–6381. doi: 10.4049/jimmunol.1102611

FIGURE 1.

FIGURE 1

TCR loci and gene targeting strategies. (A) Generation of the DJEαKI allele. Top, Tcrb locus, including relative positions of and distances between the V, D, J and C gene segments and cis-elements. Below, EαKI allele, targeting construct and DJEαKI allele. Right, Southern blot analysis of genomic DNA from wild-type (+/+), heterozygous (+/DJEαKI) and homozygous (DJEαKI/DJEαKI) mice. DNA was digested with EcoRV and hybridized with a Vβ13 probe. Expected wild-type and DJEαKI fragments are 7.1 kb and 3.5 kb, respectively. (B) Generation of the β-in-α allele. Top, Tcra/d locus, including relative positions of and distances between the V, D, J and C gene segments and cis-elements. Below, TCRαsJ allele, targeting construct and β-inα allele. Right, Southern blot of genomic DNA from wild-type (+/+), heterozygous (+/βin-α) and homozygous (β-in-α/β-in-α) mice. DNA was digested with EcoRI and hybridized with a 3′ TCRα (Cα) probe. Expected wild-type and β-in-α fragments are 3.8 kb and 5.9 kb, respectively. V, EcoRV; E, EcoRI, B, BamHI; DT, diphtheria toxin; bent arrow, promoter; open and filled large triangles, 23 bp and 12 bp RSSs, respectively; small triangles, loxP sites.