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Order parameters 〈P2d〉, 〈P2〉, and 〈P4〉 for the orientation of BR probes on the A, B, C, and D helices of smRLC in relaxed, active, and rigor states of permeabilized muscle fibers. Mean ± SE for 9 fibers, A-helix; 7 fibers, B-helix; 4–8 fibers, C-helix; and 10 fibers, D-helix. ∗ denotes active and rigor values for which differences from relaxed are significant at the 5% level as determined by a t-test.
