Table 1.
ATPase rates of scallop myofibrils containing different RLC isoforms
| −Ca2+(s−1) | +Ca2+(s−1) | +Ca2+/−Ca2+ | |
|---|---|---|---|
| Control myofibrils | 0.03 ± 0.02 | 1.06 ± 0.54 | 35 ± 27 |
| Desensitized myofibrils | 0.57 ± 0.20 | 0.48 ± 0.18 | 0.8 ± 0.3 |
| skRLC | 0.42 ± 0.06 | 0.43 ± 0.10 | 1.0 ± 0.4 |
| Scallop RLC | 0.27 ± 0.20 | 1.09 ± 0.38 | 4.0 ± 1.3 |
| BR-smRLC-A | 0.19 ± 0.14 | 1.26 ± 0.35 | 6.6 ± 4.1 (3.4 ± 1.6) |
| BR-smRLC-B | 0.54 ± 0.27 | 1.63 ± 0.60 | 3.0 ± 1.9 (4.8 ± 0.6) |
| BR-smRLC-D isomer 1 | 0.36 ± 0.09 | 1.23 ± 0.53 | 3.4 ± 0.9 (3.1 ± 2.0) |
| BR-smRLC-D isomer 2 | 0.21 ± 0.08 | 0.72 ± 0.22 | 3.4 ± 0.8 (3.3 ± 0.9) |
Mean values (± SD) for n = 6 assays in each case from a single preparation. Data in brackets in the right column are for assays (n = 4 or 5 in each case from a single preparation) of the smRLC before labeling with BR-I2.