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. Author manuscript; available in PMC: 2012 Mar 21.
Published in final edited form as: J Control Release. 2011 Nov 26;158(2):293–303. doi: 10.1016/j.jconrel.2011.11.024

Figure 2.

Figure 2

Agarose gel retardation assay of peptide/siRNA binding and nuclease protection study. Peptide/siRNA complexes were prepared at different ratio (w/w). For nuclease protection assay, the complexes were incubated with ribonuclease A at 37°C for 30 minutes. The enzyme activity was stopped by ribonuclease inhibitor and the complexes were dissociated using 2 mM SDS. Untreated siRNA, siRNA treated with nuclease and siRNA treated with SDS were served as control. Electrophoresis was carried out at 100V for 20 minutes and the gel was stained with GelRed™.