Figure 2. Impedance images of a single cell and model simulation.
The simultaneously recorded optical transmission (a), SPR (b) and EIM (c) images of a SiHa cell at 0 (1), 30 (2) and 75 (3) min after apoptosis treatment. Schematic illustration of SPR imaging (d) showing that evanescent field localized near the surface interacts with the bottom portion of a cell. The evanescent wave here is an exponential-decaying wave at the glass-gold interface. Simulated EIM (f) and SPR (e) images that reproduce the main features observed in the experimental images. Note that the dashed circles outline the cell nucleus, and the black arrows point the nucleolus of the cell. Gray scale-bar is the z-axis scale of SPR with the unit of RU. Color scale-bar is the z-axis scale of EIM with the unit of pS/pixel. The size of a single pixel is about 0.0625 µm2 (0.25µm × 0.25µm).