Figure 3. Monitoring single cell apoptosis with EIM.
A sequence of snapshots of apoptotic events in SiHa cells illustrates the time-dependent SPR (a) and EIM images (b). Bright field optical images before (c) and after (h) apoptosis are presented to show the morphology change. Quantitative profiles of SPR (d, e) and EIM (f, g) are analyzed for individual cells (d, f) and sub-cellular regions (e, g) within a single cell. Gray scale-bar is the z-axis scale of SPR with the unit of RU. Color scale-bar is the z-axis scale of EIM with the unit of pS/pixel. The size of a single pixel is about 0.0625 µm2 (0.25µm × 0.25µm).