Figure 4. Monitoring single cell electroporation with EIM.
Time-dependent SPR (a) and EIM images (b) during an electroporation process. Bright field optical images before (c) and after (h) electroporation are presented to show the morphology change. Quantitative profiles of SPR (d, e) and EIM (f, g) are analyzed for individual cells (d, f) and sub-cellular regions (e, g) within a single cell. R1 and R2 mark two regions within the cell. Gray scale-bar is the z-axis scale of SPR with the unit of RU. Color scale-bar is the z-axis scale of EIM with the unit of pS/pixel. The size of a single pixel is about 0.0625 µm2 (0.25µm × 0.25µm).