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. 2012 Feb 14;23(3):485–499. doi: 10.1021/bc200551b

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Copyright © 2012 American Chemical Society

This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.

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Titers of antibodies against mPEG and against HO-PEG were determined by direct ELISAs of sera from groups of three rabbits immunized with either mPEG₁-IFN-α (A) or HO-PEG₁-IFN-α (B). (A) Percentages of maximal binding to mPEG-SOD (filled symbols, solid curves) and to HO-PEG-SOD (open symbols, dashed curves) and the ratio of titers on mPEG/HO-PEG (“relative titers”) are indicated for three rabbits immunized with mPEG₁-IFN-α. Data for each of rabbits R8990, R9002, and R9003 are shown in green, black, and orange, respectively. (B) Percentages of maximal binding to mPEG-SOD (filled symbols, solid curves) and to HO-PEG-SOD (open symbols, dashed curves) and the ratio of titers on mPEG/HO-PEG are indicated for three rabbits immunized with HO-PEG₁-IFN-α. Data for each of rabbits R8991, R8995, and R8997 are shown in blue, red, and gray, respectively.