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. 2012 Feb 14;23(3):485–499. doi: 10.1021/bc200551b

Figure 5.

Figure 5

Washing the assay plate with Tween 20 or Tween 80, detergents that contain HO-PEG, decreased the titers detected by direct ELISAs of sera from rabbits immunized with HO-PEG-uricase (○, ●) or mPEG-uricase (Δ, ▲) and amplified the differences between the titers of anti-PEG antibodies detected in sera from these two rabbits. Direct ELISAs with mPEG-SOD as the antigen were performed as described in Experimental Procedures, except that the PBS used to wash one of the replicate assay plates before and after the addition of the enzyme-linked secondary antibody contained either 0.05% (v/v) Tween 20 (A) or 0.1% (v/v) Tween 80 (B). Higher titers were detected in the absence of detergent (filled symbols, solid curves) than in the presence of Tween (open symbols, dashed curves). The effects of each type of Tween on the results for the individual rabbits immunized with either HO-PEG-uricase or mPEG-uricase are shown by red arrows and black arrows, respectively. The ratios of titers detected in sera from these two rabbits in the presence or absence of Tween are shown by blue arrows.