MAGEA2 is growth promoting in head and neck squamous cell carcinoma (HNSCC) and normal oral keratinocyte cell lines. A, Transient transfection of MAGEA2 construct into a spontaneously immortalized oral keratinocyte cell line (NOK-SI) (at 72 hours, there was a mean [SD] 34% [3%] increase in anchorage-dependent [AD] growth). B, Small-interfering RNA (siRNA)–mediated MAGEA2 knockdown in the HNSCC cell line JHU-O11 (at 72 hours, there was a mean [SD] 28% [3%] decrease in AD growth). Error bars represent SEM. Error bars for some data points are too small to be seen around the data point marker. CCK-8 indicates Cell Counting Kit-8; EV, empty vector.