Fig. 1.
Purification and phosphosite analysis of syndapin I from 32P-labeled rat brain synaptosomes. (A) Syndapin I (Sdpn I) was immunoprecipitated (IP) from 32P-labeled rat brain synaptosome lysates. Proteins stained with Coomassie Blue, and autoradiography detected phosphorylated syndapin I (Phospho-Sdpn I). The quadruplicate samples are representative of three independent experiments. (B) Summary of two phosphosites identified in syndapin I by mass spectrometry (precursor mass/charge and peptide mass) followed by Mascot database searches (score and expect). The confirmed phosphosite in each peptide is bolded.