Fig. 5.

In-cell membrane tubulation analysis of syndapin I F-BAR phosphosite mutants. (A–K) Membrane tubulation was analyzed by overexpressing GFP-syndapin I F-BAR-WT (SdpnI F-BAR-WT), the phospho-mutants and lipid-binding mutants in COS7 cells. Nuclei were stained blue with DAPI to show the position of the cells. GFP-FBP17 F-BAR was a positive control for tubulation (A). The images are representative of at least four independent experiments. Scale bar, 20 μm. (L) Quantitative representation of data from experiments in A–K. One hundred cells per experiment overexpressing each GFP- construct were scored for tubulation and results presented as a % of total cell count with tubulation, n = 4 ± SEM. One-way ANOVA was applied: ∗∗  ∗ P < 0.0001 against WT, ###P < 0.0001 against A mutants.