Fig. 1.

Dicer-Pten DKO mice develop high-grade metastatic serous carcinoma. (A) Severe ascites in an 8.4-mo-old DKO mouse. (B) Early tumors form in the fallopian tube (yellow arrows) of a 5-mo-old DKO mouse with normal ovaries (white arrowheads). (C) Progression of the fallopian tube tumors in a DKO mouse at 8 mo. Ovaries are still intact (white arrowheads). (D) Bilateral fallopian tube/ovarian tumors are observed in a DKO mouse at 6 mo. (E) The DKO mouse described in A showing extensive peritoneal metastasis with clusters of tumor nodules (yellow arrows) and a massive accumulation on the diaphragm (green arrows), besides fallopian tube/ovarian tumors (black arrows). (F) Survival curve of DKO and control mice. (G–I) DKO fallopian tube/ovarian tumors showing papillary structure and irregular glands with slit-like spaces, characteristic of high-grade serous carcinoma (H&E) and representative of fallopian tube/ovarian tumors from 16 DKO mice. (H–J) The nuclear features of high-grade serous carcinomas including nuclear pleomorphism (red arrow), prominent nucleoli with irregular chromatin patterns (green arrow), apoptosis (black arrow), and brisk mitotic activity (yellow arrow) (H&E). (J) Fallopian tube/ovarian tumor with a solid growth pattern (H&E). Magnifications: G, 20×; H–J, 40×.)