Fig. 2.

Analyses of aP1•aEF-2 binding using the mutant proteins. (A and B) Homodimers of the aP1 point mutants, Leu106Ser (A) and Phe107Ser (B) (200 pmol each), were incubated without aEF-2 (lane 1) or with 400 pmol (lane 2), 800 pmol (lane 3), 1.2 nmol (lane 4), or 1.6 nmol (lane 5) of aEF-2. aEF-2 (400 pmol) was also incubated alone (lane 6). (C) 200 pmol of wild-type aP1 were incubated with domain I of aEF-2 as in A and B. Gel analysis was carried out as in Fig. 1.