Fig. 2.

Lymphocyte development defects in 53BP1^−/−XLF^−/− mice are due to impaired V(D)J recombination. (A) Representative flow cytometric analyses of bone marrow and spleen from WT, XLF^−/−53BP1^−/−, 53BP1^−/−XLF^−/−HL, and 53BP1^−/−XLF^−/−EμBcl2+ mice. Numbers on the plot are percentage of total live cells (determined by forward and side scatter) represented by indicated population. (B) The number of total DN and single-positive (SP) thymocytes and IgM⁺ splenic B cells were calculated based on total organ cellularity and the percentage of the given cell type (by flow cytometric analyses). Each value listed represents the average ± SD from at least three mice of each genotype between 4 and 12 wk of age. The P values are calculated between corresponding groups by using two-tailed Student's t test. The original data are provided in Fig. S2.