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. 2012 Feb 21;109(10):3903–3908. doi: 10.1073/pnas.1120160109

Fig. 3.

Fig. 3.

53BP1−/−XLF−/− B cells have end joining during chromosomal V(D)J recombination. (A and D) Schematic of the pMX-INV (A) and pMX -SJ (B) retroviral V(D)J recombination substrates (13). The diagrams illustrate the unrearranged substrate (UR), CE/SE intermediates, and CJ/SJ products. The RSS (open and filled triangles), GFP, IRES-truncated hCD4 (ihCD4), and the LTRs are marked. The positions of EcoRV (E) sites and NcoI (N) sites are shown. hCD4 probe is located within ihCD4. (B and C) Southern blot analysis with hCD4 probe of EcoRV+NcoI (Upper) and EcoRV (Lower) digested genomic DNA from the indicated v-abl–transformed B cells containing pMX-INV substrates treated with STI571 for 2 or 4 d with or without ATM kinase inhibitor (15 μM). (E) Southern blot analysis with hCD4 probe of EcoRV digested genomic DNA from the indicated lines containing integrated pMX-SJ substrate The results shown in B and E were obtained from pools of B-cell lines with diverse integration sites of given substrate. C showed the result of cell line with single clonal integration of pMX-INV and the corresponding clones with ectopic expression of XLF.