Fig. 3.

Intravenous injection of a polyclonal anti-TFPI antibody mitigates bleeding in F8^−/− mice. (A) Plasma FXa activity in samples from F8^−/− mice treated with different i.v. doses of polyclonal anti-mouse TFPI antibody or a vehicle control shows essentially total inhibition of TFPI activity at all doses of antibody tested. Data are presented as mean ± SD. Max represents data for plasma samples obtained from vehicle control-treated mice that were spiked with 100 μg/mL of the anti-mouse TFPI antibody and is the maximum total inhibition of plasma TFPI activity. (B) Treatment of F8^−/− mice with increasing doses of a polyclonal anti-mouse TFPI antibody produces progressively smaller amounts of blood loss following 4-mm tail transection (test for linear trend, r² = 0.40, P = 0.000024).