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. 2012 Feb 6;109(10):E613–E621. doi: 10.1073/pnas.1115029109

Fig. 3.

Fig. 3.

Akt1 selectively relieves the repression of ISGs via EMSY phosphorylation at Ser209. (A) Western blots of lysates of HMEC-tert cells transduced with retroviral constructs of MyrAkt1, MyrAkt2, or EV were probed with the indicated antibodies. (B) Expression of IFITM1, ISG15, and Viperin was examined in the same cells as in A by real-time RT-PCR. Data represent fold induction of these genes in MyrAkt1- and MyrAkt2-expressing cells relative to their expression in EV cells. Error bars show the SD (n = 3). (C) Immunoprecipitates of lysates derived from HMEC-tert cells transduced with retroviral constructs of MyrAkt1, MyrAkt2, or EV and superinfected with pLenti constructs of WT EMSY or EMSY S209A were probed with the indicated antibodies. IP, immunoprecipitate; IB, immunoblot. (D) Expression of IFITM1, ISG15, and Viperin was examined in the same cells as in C by real-time RT-PCR. Data are represented as fold change relative to EV. Error bars show the SD (n = 3). Western blotting of lysates from the same cells is also shown.