Fig. 3.

Stable increased WIP1 expression failed to affect growth of TP53 mutant medulloblastoma cells. (A) Western blotting of whole cell lysates from TP53 mutant Daoy clones with stable expression of an empty vector (pcDNA), of WIP1, or of a mutated, phosphatase-deficient WIP1 (WIP1 D314A). (B) Scatter plot of quantified WIP1 expression, relative to β-actin and to expression in the Daoy empty-vector (Daoy-pcDNA) clone 2C. Error bars represent standard error of the mean. (C) Cell viability assayed by trypan blue exclusion in adherent cultures of Daoy clones 24–72 h following initial plating of cells (*P = .004 at 72 h). Error bars represent standard deviation among triplicates.