Fig. 4.

Developmental expression of Aq-Cry2. (A,B,D,K) Cleared whole-mounts; (C,E,G,J) whole-mounts; (F,H,I,L) sections; (C,E,G) posterior pole facing up; (D,F,H–L) posterior pole to right. (A,B) Aq-Cry2 expressing cells are scattered throughout the embryo during cleavage. (C–E) In later stages, Aq-Cry2 transcripts are predominantly detected in the vicinity of the pigment spot and early ring. (F,G) During ring formation, Aq-Cry2 expression continues outside, underneath and inside the ring, and also becomes evident in a population of cells located beneath the outer-layer, putative sub-epithelial cells. (H–J) As the embryo develops into an early larva, expression of the gene increases in the outer layer, appearing strongest at the anterior and posterior poles. (I) Higher magnification of the embryo shown in H. (K,L) In swimming larvae, Aq-Cry2 is expressed in sub-epithelial cells, epithelial-like cells, pigment cells, cells with long cilia and in a subset of cells located inside the pigment ring.