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. 2012 Mar 21;7(3):e34004. doi: 10.1371/journal.pone.0034004

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Yuan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) DACT1 mRNA levels were assayed using quantitative real-time RT-PCR analysis. Samples of colon adenocarcinoma (T, white bars) and normal-appearing control mucosa (N, black bars) were analyzed in six cases of colon cancer. (B) Expression of DACT1 protein in human colon adenocarcinoma (T) and normal-appearing control mucosa (N) in six cases, as determined by Western immunoblotting analysis. The expression of GAPDH was used as the loading control.