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. 2012 Mar 21;7(3):e34004. doi: 10.1371/journal.pone.0034004

Figure 6. DACT1 enhances the migratory and invasive potential of colon cancer cells through changing the subcellular location of β-catenin.

Figure 6

(A) Photomicrographs of empty vector and DACT1-overexpressing SW480 cells immunostained with an anti-β-catenin antibody (red). (B, C, D) Photomicrographs of control siRNA and DACT1 siRNA in HCT116, LoVo and HT29 cells immunostained with an anti-β-catenin antibody (red). (E) Representative blots of β-catenin levels in membrane (Mem), nuclear (Nuc), and cytoplasmic (Cyto) fractions and total lysates (Lys) in SW480 cells. Laminin B (nuclear expression) and GAPDH (cytoplasmic expression) were used as the loading controls. “+” represents the overexpression DACT1. “−” is empty vector. (F) Representative blots of β-catenin levels in membrane (Mem), nuclear (Nuc), and cytoplasmic (Cyto) fractions and total lysates (Lys) in HCT116 cells. Laminin B (nuclear expression) and GAPDH (cytoplasmic expression) were used as the loading controls. “+” represents control siRNA. “−” is DACT1 siRNA.