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. 2012 Mar 21;7(3):e34004. doi: 10.1371/journal.pone.0034004

Figure 7. Correlation between DACT1 levels and membrane-associated β-catenin expression in colon cell lines and colon cancer tissue.

Figure 7

(A) Semiquantitative RT-PCR analysis of DACT1 expression in HCT116, HT29 and SW480 cells. GAPDH was used as the control. (B) Immunofluorescence analysis of DACT1 and β-catenin expression in HCT116, HT29 and SW480 cells. Original magnification, 40×. (C, D) HE staining of samples of normal human colon mucosa (N) and adenocarcinoma (T) tissues by an anti-β-catenin antibody. Original magnification, 40×. (E, F) immunofluorescence (IF) staining of samples of normal human colon mucosa (N) and adenocarcinoma (T) tissues by an anti-β-catenin antibody. Original magnification, 40×. (G, H) Immunohistochemical (IHC) staining of samples of normal human colon mucosa (N) and adenocarcinoma (T) tissues by an anti-β-catenin antibody. Original magnification, 40×.