Table 2.
Results of mass spectrometry identification
| Protein name | Gene name | Swiss-Prot ID | pI/MW | Frequency1 | Fold2 | P value | Coverage3 | No. of pep.4 |
| Down-regulated | ||||||||
| Gastrokine-1 precursor | GKN1 | Q9NS71 | 5.3/21.0 | 75% (9/12) | 15.17 | 0.000 | 16% | 6 |
| 15-hidroxyprostaglandin dehydrogenase isoform 1 | HPGD | P15428 | 5.6/25.2 | 66% (8/12) | 3.85 | 0.016 | 23% | 5 |
| Enoyl CoA hydratase, mitochondrial precursor | ECHS1 | P30084 | 5.8/27.9 | 66% (8/12) | 3.38 | 0.001 | 49% | 15 |
| 39S ribosomal protein L12, mitochondrial precursor | MRPL12 | P52815 | 5.1/20.6 | 58.3% (7/12) | 10.95 | 0.016 | 16% | 4 |
| Acyl-CoA dehydrogenase | ACADS | P16219 | 6.1/39.9 | 58.3% (7/12) | 4.94 | 0.005 | 12% | 5 |
| NADH dehydrogenase (ubiquinone) flavoprotein 2, mitochondrial precursor | NDUFV2 | P19404 | 5.7/24.2 | 50% (6/12) | 2.26 | 0.027 | 58% | 16 |
| Phenazine biosynthesis-like domain-containing protein isoform a | PBLD | P30039 | 6.1/31.8 | 50% (6/12) | 3.60 | 0.007 | 25% | 5 |
| Plasma cell-induced resident endoplasmic reticulum protein | PACAP | Q8WU39 | 5.2/22.0 | 41.6% (5/12) | 4.41 | 0.042 | 39% | 5 |
| ATP synthase subunit d, mitochondrial | ATP5H | O75947 | 5.2/20.5 | 41.6% (5/12) | 2.40 | 0.012 | 26% | 5 |
| Glutathione S-transferase mu 3 | GSTM3 | P21266 | 5.3/26.3 | 33.3% (4/12) | 3.35 | 0.042 | 29% | 4 |
| Transthyretin | TTR | P02766 | 5.4/16.4 | 25% (3/12) | 2.74 | 0.027 | 53% | 7 |
| Up-regulated | ||||||||
| Septin-2 | SEPT2 | Q15019 | 6.0/41.0 | 75% (9/12) | 4.58 | 0.009 | 34% | 7 |
| Maspin | SERPINB5 | P36952 | 5.6/40.2 | 58.3% (7/12) | 4.99 | 0.007 | 14% | 5 |
| Phosphoglycerate kinase 1 | PGK1 | P00558 | 6.0/40.1 | 58.3% (7/12) | 6.46 | 0.016 | 22% | 6 |
| Pyruvate kinase, muscle | PKM2 | P14619 | 6.0/38.8 | 58.3% (7/12) | 5.25 | 0.021 | 20% | 8 |
| Actin, cytoplasmic 1 | ACTB | P60709 | 4.8/32.2 | 58.3% (7/12) | 7.77 | 0.005 | 44% | 12 |
| Tumor necrosis factor receptor superfamily member 16 precursor | NGFR | P08138 | 5.2/35.5 | 50% (6/12) | 3.94 | 0.002 | 17% | 5 |
| Heat shock 70 kDa protein 1A/1B | HSPA1 | P08107 | 5.1/36.9 | 50% (6/12) | 4.77 | 0.009 | 14% | 8 |
| Heterochromatin-like protein 1 | CBX3 | Q13185 | 5.2/22.0 | 50% (6/12) | 4.12 | 0.027 | 19% | 3 |
| Ubiquitin-conjugating enzyme E2 N | UBE2N | P61088 | 5.7/16.4 | 41.6% (5/12) | 4.94 | 0.001 | 40% | 5 |
| Transaldolase | TALDO1 | P37837 | 5.9/38.5 | 41.6% (5/12) | 3.12 | 0.003 | 18% | 5 |
| Cathepsin D precursor | CTSD | P07339 | 5.3/28.4 | 41.6% (5/12) | 3.49 | 0.034 | 27% | 11 |
| Annexin A4 | ANXA4 | P09525 | 5.6/31.3 | 41.6% (5/12) | 13.31 | 0.027 | 54% | 26 |
| Myotrophin | MTPN | P58546 | 5.1/13.5 | 41.6% (5/12) | 2.45 | 0.000 | 22% | 4 |
| Enolase 1 variant | ENO1 | P06733 | 5.5/42.6 | 41.6% (5/12) | 3.95 | 0.034 | 19% | 7 |
| Nicotinamide N-methyltransferase | NNMT | P40261 | 5.4/28.5 | 41.6% (5/12) | 4.24 | 0.000 | 9% | 3 |
| Eukaryotic translation initiation factor 2 subunit 1 | EIF2S1 | P05198 | 4.9/33.5 | 41.6% (5/12) | 6.61 | 0.042 | 24% | 8 |
| Translationally-controlled tumor protein | TPT1 | P13693 | 4.8/20.7 | 25% (3/12) | 2.50 | 0.002 | 18% | 4 |
| Annexin A5 | ANXA5 | P08758 | 5.0/32.4 | 25% (3/12) | 3.24 | 0.005 | 70% | 25 |
| Nucleoside diphosphate kinase A isoform a | NME1 | P15531 | 5.7/19.0 | 25% (3/12) | 3.11 | 0.016 | 33% | 5 |
1
Frequency shows the number of patients where the protein was differentially expressed in two-dimensional gel electrophoresis analysis;
2
Fold stands for the average fold change in the relative spot volume between non-tumor and tumor (downregulated) or tumor and non-tumor (upregulated) tissue;
3
Coverage stands for the sequence coverage in mass spectrometry (MS) analysis;
4
No. of pep. stands for the number of matched peptides in MS analysis. MW: Molecular weight.