Table 1.
Primers used for end-point RT-PCR
| Gene of Interest | Primer | Sequence (5'-3') |
|---|---|---|
| BMP2 | Forward Reverse |
CTTAGACGGTCTGCGGTCTC CGAAGCTCTCCCACCTACTG |
| BMP4 | Forward Reverse |
TGAGCCTTTCCAGCAAGTTT TACGATGAAAGCCCTGATCC |
| NOG | Forward Reverse |
GAACACCCGGACCCTATCTT ATGGGGTACTGGATGGGAAT |
| BMPRII | Forward Reverse |
AGACTGTTGGGACCAGGATG GTCTGGCCCACTGAATTGTT |
| ACVR1 | Forward Reverse |
AAATGGGATCGCTGTACGAC CTGTGAGTCTGGCAGATGGA |
| BMPR1A | Forward Reverse |
CAGGTTCCTGGACTCAGCTC CACACCACCTCACGCATATC |
| BMPR1B | Forward Reverse |
AGGTCGCTATGGGGAAGTTT CTCCCAAAGGATGAGTCCAA |
| ACTBa | Forward Reverse |
CTGTCCCTGTATGCCTCTGG AGGAAGGAAGGCTGGAAGAG |
a[15]