Table 3.
Number of cells harvested, number of population doublings and doubling time for each BMSC passage
| T-75 flasks | 2-layer cells factories | First culture in 10-layer cell factories | Second culture in 10-layer cell factories | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Donation Number | Donor | CFE | Yield (×106) | Yield (×106) | Population Doublings | Doubling Time (hr) | Yield (×109) | Population Doublings | Doubling Time (hr) | Yield (×109) | Population Doublings | Doubling Time (hr) | Cumulative Population Doublings | Units in Storage |
| W092110086001 | 1 | 6 | 6.5 | 94.4 | 3.9 | 30.9 | 0.81 | 3.2 | 37.3 | 1.73 | 3.4 | 34.9 | 10.5 | 10 |
| W092110086002 | 2 | 18 | 30.3 | 372 | 4.5 | 26.4 | 0.99 | 3.6 | 33 | 2.43 | 3.9 | 30.6 | 12 | 19 |
| W092110086003 | 3 | 9 | 21 | 314.3 | 4.3 | 27.9 | 1.28 | 4 | 30 | 1.61 | 3.3 | 36 | 11.6 | 14 |
| W092110086004 | 4 | 0 | 0.2 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
| W092110086005 | 5 | 11.5 | 52.1 | 456.4 | 4.8 | 24.8 | 1.23 | 3.9 | 30.4 | 4.76 | 4.9 | 24.5 | 13.6 | 40 |
| W092110086006 | 7 | 15 | 28 | 372.5 | 4.5 | 26.4 | 0.9 | 3.5 | 34.3 | 2.17 | 3.8 | 31.9 | 11.8 | 20 |
| W092110086007 | 10 | 1.5 | 25.4 | 255.4 | 4 | 30 | 1.1 | 3.8 | 31.8 | 1.66 | 3.4 | 35.6 | 11.2 | 15 |
| W092111086001 | 11 | 7.5 | 3.9 | 27.9 | 3.9 | 30.9 | 0.15 | 2.9 | 49.8 | 0.99 | 3 | 47.2 | 8.8 | 9 |
| W092111086002 | 1 | 7 | 3.1 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
| W092111086003 | 14 | 5.5 | 12.9 | 233.2 | 4.3 | 28 | 0.97 | 3.6 | 33.3 | 2.52 | 3.98 | 30.2 | 11.9 | 23 |
| W092111086004 | 15 | 4.5 | 2.2 | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
NA not applicable; CFE colony formation efficiency