Table 3.
List of strains used in this study and their genotypes
| Strain | Genotype | Plasmid | Source |
|---|---|---|---|
| E. coli strains | |||
| E. coli DH5α | supE44 lacU169 (ϕ80lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | ||
| S. cerevisiae strains | |||
| CEN.PK 113-5D | MATa MAL2-8c SUC2 ura3-52 | P. Kötter, University of Frankfurt, Germany | |
| CB0 | CEN.PK 113-5D | pSP-GM2 | This study |
| CB1 | CEN.PK 113-5D | pSP-GM2 carrying WS gene from A. baylyi ADP1 | This study |
| CB2 | CEN.PK 113-5D | pSP-GM2 carrying WS gene from M. hydrocarbonoclasticus DSM 8798 | This study |
| CB3 | CEN.PK 113-5D | pSP-GM2 carrying WS gene from R. opacus PD630 | This study |
| CB4 | CEN.PK 113-5D | pSP-GM2 carrying WS gene from Mus musculus C57BL/6 | This study |
| CB5 | CEN.PK 113-5D | pSP-GM2 carrying WS gene from P. arcticus 273-4 | This study |
| CB2A | CEN.PK 113-5D | pSP-GM2 carrying WS gene from M. hydrocarbonoclasticus DSM 8798 and ACC1 | This study |
WS: wax ester synthase