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. 2012 Mar 21;7(3):e32923. doi: 10.1371/journal.pone.0032923

Figure 5. Phylogenetic reconstruction of the evolutionary relationship among vertebrate RXFP3/4 protein sequences.

Figure 5

Reconstruction performed as outlined in methods with Gā€Š=ā€Š0.91 and Iā€Š=ā€Šn/a. Numbers at each node indicate the bootstrap values (only values exceeding 50% shown). Teleost rxfp3-2 underwent duplication yielding two 3R-paralogs, rxfp3-2a and rxfp3-2b, while teleostean ancestral rxfp3-3 was duplicated giving rise to typically three rxfp3-3 loci in modern teleosts: 3R generated rxfp3-3a and rxfp3-3b, while a local duplication generated rxfp3-3a1 and rxfp3-3a2. Solely in zebrafish, rxfp3-3a2 duplicated again giving rise to rxfp3-3a3, an event which appears to have occurred coincidently with the exclusive loss of rxfp4 in zebrafish.